Mounting
microscope slides refers to the coverslip mounting
procedure. There are a number of different methods
for mounting tissue sections. Glycerol can be
used for temporary mounting. If a more permanent
seal is important, Permount is recommended. Permount
will not discolor with age.
note:
work under fume hood
1.
dehydrate tissue sections through graded alcohol
series and toluene (xylene)
2. remove the slide from toluene
3. place the slide horizontaly - tissue section
facing upwards
4. apply 1-2 drops of permount onto the tissue
section
5. gently place the coverslip onto the tissue
sample
6. using forceps, apply slight pressure on the
coverslip - eliminate any air bubbles
7. using tissue paper, blot any excess permount/toluene
from around the edges of the coverslip
8. keep the slides flat; allow overnight drying
(a slide oven at 75 C can be used as well)
9. although permount is permanent, coverslips
can be removed by soaking slides in toluene
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