| Immhunohistochemistry
Terms and Definitions |
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antigen
antigens
are typically proteins, however, they can be any
substance that triggers an immune response and
antibody production
antigen
retrieval is a protocol to expose antigens masked
by fixation (see antigen
retrieval protocol)
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avidin
- biotin complex
there
is a high affinity between avidin and biotin;
immunohistochemistry makes use of this high affinity
by using biotinylated antibodies to amplify tissue
antigen signals
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counterstain
after
immunohistochemical processing, counterstains
are often applied to better visualize the results
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cryosection
cryosectioning
is a tissue sectioning technique that cuts frozen
tissue using a cryostat (a microtome operating
at approximately -25 C)
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dewax
the
process of removing paraffin from paraffin embedded
tissues
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fixative
a solution
that helps preserve tissue and cell morphology
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IgG
immunoglobulin
G - an antibody type normally present in the blood
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IgM
immunoglobulin
M - one of the 5 classes of immunoglobulins
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primary
antibody
primary
antibodies are glycoproteins that specifically
bind antigens (epitopes)
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secondary
antibody
a secondary
antibody is raised to the Ig of the species from
which comes the primary antibody; the secondary
antibody will label the primary antibody
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triton
triton
(triton x-100) is a nonionic surfactant; it is
used to permeabilize cell memnranes to allow greater
access to antibodies
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